PCR-RFLP technique has been used to detect species-specific mutations of organelles DNA for closely related dwarf mountain pine (Pinus mugo) and Scots pine (P. sylvestris). Restriction fragment patterns have been compared of amplification products for trnL-trnF cpDNA and for coxI and orf25 genes of mtDNA. https://www.aitsglobal.com/product-category/grits/